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Restriction enzymes, modifying enzymes, buffering solutions, inhibitors, and substrates for use in clinical, research, and general laboratory procedures.
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A component of the Papain Dissociation System. This material is 0.22 micron membrane filtered and lyophilized in autoclaved vials. A vial reconstituted with 0.5 ml of EBSS or equivalent yields a solution of 2000 units/ml of deoxyribonuclease (1 mg/ml).
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Deoxyribonuclease I, Chromatographically purified, Equivalent to 1X crystallized Dnase, A lyophilized powder with glycine as a stabilizer, Store at 2 to 8degreeC, Pack Size:100mg, Source: Bovine Pancreas, min. activity: 2000U/mgKunitz dry w
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Nuclease, Micrococcal (S7); Micrococcal nuclease (MN) catalyzes cleavage of both DNA and RNA to yield 3'-nucleotides; MN is the extracellular nuclease of Staphylococcus aureus; M.W. 16807; Optimum 9.2 pH; Stored: 2 deg. to 8 deg.C
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The DNase I Treatment Kit efficiently removes genomic DNA from RNA samples. It utilizes an endonuclease that non-specifically cleaves ssDNA, dsDNA and the DNA strand of RNA:DNA hybrids to improve the purity of your RNA samples. Following DNase I treatment, RNA samples are purified with easy-to-use spin columns. The RNA is then suitable for use in downstream applications such as RT-qPCR and RNA-seq. For more complete information, please visit the Active Motif website.
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CircLigase™ ssDNA Ligase is a thermostable ATP-dependent ligase that catalyzes intramolecular ligation (i.e. circularization) of ssDNA templates having a 5-phosphate and a 3-hydroxyl group
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Taq DNA Ligase catalyzes the formation of a phosphodiester bond in duplex DNA containing adjacent 5′-phosphoryl and 3′-hydroxyl termini, using NAD+ as a cofactor. The ligation will occur only if the oligonucleotides are perfectly paired to the complementary target DNA and have no gaps between them; therefore, a single-base substitution can be detected. This product is active at elevated temperatures (45°C-70°C) (1, 2).Applications Allele-specific gene detection by using Ligase Detection Reaction (LDR) and Ligase Chain Reaction (LCR) (1). Mutagenesis by incorporation of a phosphorylated oligonucleotide during primer extension amplification(3).
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Intact Genomics (IG) PEmax enzyme is the purified recombinant Streptococcus pyogenes Cas9 nickase mutant (H840A)-Murine Moloney Leukemia Virus-Reverse Transcriptase (MMLV-RT) fusion protein3-4 containing a nuclear localization signal (NLS) at the C-terminal for targeting to the nucleus. This enzyme is designed to perform CRISPR prime-editing3-4. The physical purity of this enzyme is ≥85% as assessed by SDS-PAGE with Coomassie blue staining.Product Includes: PEmax enzyme 5x PEmax Nicking Buffer: 20 mM HEPES, 100 mM NaCl, 5 mM MgCl2, 0.1 mM EDTA, pH 6.5 @ 25 °CStore all components at -20°C.
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Boc-Gln-Arg-Arg-AMC acetate is a fluorogenic substrate utilized for determining protease activity. Upon hydrolysis, it releases the fluorescent product 7-amino-4-methylcoumarin (AMC), which emits a fluorescent signal under UV light. This product is for research use only.
Fluorogenic substrate
Used for determining protease activity
Releases fluorescent product 7-amino-4-methylcoumarin (AMC) upon hydrolysis
Emits fluorescent signal under UV light
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